{"product_id":"beclin-1-polyclonal-antibody-bs79694","title":"Beclin 1 Polyclonal Antibody-BS79694","description":"\u003cp\u003eBeclin 1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS79694-50, BS79694-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q14457\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eBeclin 1 Polyclonal Antibody-BS79694\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP,\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000  |  IP,50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 52KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using Beclin 1 Rabbit pAb at 1:1000 dilution.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 30s., Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Beclin 1 antibody. Western blot was performed from the immunoprecipitate using Beclin 1 antibody at a dilition of 1 :1000.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: ATG6; VPS30; beclin1; Beclin 1; BECN1; beclin-1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human Beclin 1(NP_003757.1).\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42960119496883,"sku":"BS79694-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42960119529651,"sku":"BS79694-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_40105f50-6680-4aaf-8a75-ceb4bf864fec.jpg?v=1705231719","url":"https:\/\/afsbio.com\/products\/beclin-1-polyclonal-antibody-bs79694","provider":"AFSBio Inc.","version":"1.0","type":"link"}