{"product_id":"beta-actin-polyclonal-antibody-e-ab-40338","title":"beta actin Polyclonal Antibody - E-AB-40338","description":"\u003cp\u003ebeta actin Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 60μL, 120μL, 200μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-AB-40338-60, E-AB-40338-120, E-AB-40338-200\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: ACTBB\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: ACTB; Actin beta; Actx; Actin cytoplasmic 1; b actin; A26C1A\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Isotype, Tags and Cell markers, Signal Transduction\u003c\/p\u003e\n\n\u003cp\u003eConjugation: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eSpecies Reactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplication: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n \n\n\u003cp\u003eUNIProt ID: Q7ZVF9\u003c\/p\u003e\n\n\u003cp\u003eBackground: Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 0.85mg\/mL\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant Zebrafish Actin, cytoplasmic 2 protein\u003c\/p\u003e\n\n\u003cp\u003eBuffer: PBS with 0.05% Proclin300, 50% glycerol, pH7.3.\u003c\/p\u003e\n\n\u003cp\u003ePurification Method: Antigen Affinity Purification\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB 1:3000-1:30000  IHC 1:100-1:300\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 41 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 41 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: -20°C\/One year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: Ice bag\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"60μL","offer_id":43146027630771,"sku":"E-AB-40338-60","price":145.6,"currency_code":"CAD","in_stock":true},{"title":"120μL","offer_id":43146027663539,"sku":"E-AB-40338-120","price":249.2,"currency_code":"CAD","in_stock":true},{"title":"200μL","offer_id":43146027696307,"sku":"E-AB-40338-200","price":292.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_2ac1c0ea-2774-4c98-bcf9-2b6b793da253.png?v=1710290090","url":"https:\/\/afsbio.com\/products\/beta-actin-polyclonal-antibody-e-ab-40338","provider":"AFSBio Inc.","version":"1.0","type":"link"}