Bevacizumab (Avastin) Pharmacokinetic ELISA

Bevacizumab (Avastin) Pharmacokinetic ELISA

Regular price
$914.27
Regular price
Sale price
$914.27
Unit price
per 
Availability
Sold out

Product Properties

CATALOGUE NUMBER: EL-1611-021

DETECTION METHOD
Peroxidase / OD450

STORAGE AND STABILITY
Stable at -20°C for 1 year

SAFETY WARNINGS AND PRECAUTIONS
Research Use Only. Follow instructions.

PRECISION
Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%.

DETECTION LIMIT
1.5ng/ml

EACH KIT INCLUDES:
Coated microtiter plate, 96 wells
Calibrator diluent. - 1.8ml
Calibrator (250 ug/mL) - 12ul
10X wash buffer - 25ml
Assay buffer - 50ml
1000X detection reagent - 17ul
TMB - 12ml
TMB stop solution - 12ml
Plate sealers - 3

DESCRIPTION

INTRODUCTION
The Bevacizumab ELISA kit is designed to measure free Bevacizumab with high specificity and sensitivity. The assay design uses a pair of antibodies allowing detection of whole Bevacizumab molecules in biological matrices.
Bevacizumab (Trade name Avastin®) is a recombinant humanized monoclonal antibody that blocks angiogenesis by inhibiting vascular endothelial growth factor A(VEGF-A). VEGF-A is a chemical signal that stimulates angiogenesis in a variety of diseases, especially in cancer. Bevacizumab was the first clinically available angiogenesis inhibitor in the United States.

PRINCIPLE OF THE ASSAY

This assay employs the sandwich enzyme immunoassay technique. Anti-Bevacizumab is coated onto a 96 well microplate. Calibrator, quality control samples and test samples are pipetted into the appropriate wells. Bevacizumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Bevaciuzumab present in test samples.

 

Download the Specifications Here

Download the Manual Here