{"product_id":"bim-polyclonal-antibody-bs90137","title":"Bim Polyclonal Antibody-BS90137","description":"\u003cp\u003eBim Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90137-50, BS90137-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O43521(Human)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC, IHC, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB:1:1,000-1:2,000\u003cbr\u003e\nICC:1:50-1:200\u003cbr\u003e\nIHC:1:50-1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Pro-apototic Bcl-2 family members promote cell death by neutralizing their anti-apoptotic relatives, which otherwise maintain cell viability by regulating caspase activity. Bim belongs to the BH3-only subgroup of Bcl-2 related proteins, and exists in three distinct isoforms, BimS (short), BimL (long) and BimEL (extra long). ERK1\/2 phosphorylates BimEL, resulting in rapid degradation of the isoform via the proteasome pathway. At least three sites for ERK1\/2 phosphorylation exist on BimEL, whereas ERK1\/2 does not effect BimS and BimL, implying a unique role for BimEL in cell survival signaling.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Bim polyclonal antibody detects endogenous levels of Bim protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 22\/16\/13 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Bim on different lysates using anti-Bim antibody at 1\/1,000 dilution. \u003cbr\u003e\n Positive control: \u003cbr\u003e\n Lane 1: Raji \u003cbr\u003e\n Lane 2: Jurkat \u003cbr\u003e\n Lane 3: A431, ICC staining Bim in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Bcl2-L-11, BCL2L11, BCL2 like 11, B2L11, BAM, Bcl 2 interacting protein Bim, Bcl 2 related ovarian death agonist, Bcl-2-like protein 11, BCL2 interacting mediator of cell death, BCL2 like 11 (apoptosis facilitator), BCL2 like protein 11, Bcl2-interacting mediator of cell death, Bcl2-L-11, Bcl2l11, BIM alpha6, BIM, BIM beta6, BIM beta7, BimEL, BimL, BOD,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42979764142259,"sku":"BS90137-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42979764175027,"sku":"BS90137-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_06788197-91ee-4007-8a26-1b3c95e77c16.jpg?v=1705748902","url":"https:\/\/afsbio.com\/products\/bim-polyclonal-antibody-bs90137","provider":"AFSBio Inc.","version":"1.0","type":"link"}