{"product_id":"biotinylated-human-cd3e-c-fc-avi-ept267","title":"Biotinylated Human CD3E (C-Fc-Avi) - EPT267","description":"\u003cp\u003eBiotinylated Human CD3E (C-Fc-Avi)\u003c\/p\u003e\n\n\u003cp\u003eSize: 20ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: EPT267-20\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: T-Cell Surface Glycoprotein CD3 ε Chain (CD3ε) is a single-pass type I membrane protein. CD3ε contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3ε is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex (TCR\/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). TCR\/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: T-Cell Surface Glycoprotein CD3 Epsilon Chain; T-Cell Surface Antigen T3\/Leu-4 Epsilon Chain; CD3e; CD3E; T3E\u003c\/p\u003e\n\n\u003cp\u003eDescription: Biotinylated Recombinant Human T-cell Surface Glycoprotein CD3 Epsilon Chain is produced by our Mammalian expression system and the target gene encoding Asp23-Asp126 is expressed with a Fc, Avi tag at the C-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAccession: P07766\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: 40.5 KDa\u003c\/p\u003e\n\n\u003cp\u003eApparent Molecular Weight: 45-60 KDa, reducing conditions\u003c\/p\u003e\n\n\u003cp\u003eStorage Condition: Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\n\n\u003cp\u003ePurity: Greater than 95% as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: Less than 0.1 ng\/µg (1 EU\/µg) as determined by LAL test.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: Loaded Anti-Human CD3E mAb-mFc (Cat#NC079) on AMC Biosensor, can bind Human CD3E-Fc-6His\u0026amp;C-Fc-Flag (Cat#CY33) with an affinity constant of 0.6 nM as determined in BLI assay.\u003c\/p\u003e\n\n\u003cp\u003eRedissolve: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg\/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. \u003c\/p\u003e\n\n\u003cp\u003eDelivery Condition: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"Default Title","offer_id":43211050877107,"sku":"EPT267-20","price":754.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_bbfcd2bf-b904-4570-a6ca-e5eb8d8b9f84.png?v=1767117921","url":"https:\/\/afsbio.com\/products\/biotinylated-human-cd3e-c-fc-avi-ept267","provider":"AFSBio Inc.","version":"1.0","type":"link"}