1 x 500 µl Protein Marker in loading buffer, sufficient for 100 mini gels or 50 large gels
Broad range: 10 – 240 kDa
Catalogue Number: MWP04
BlueStar PLUS Prestained Protein Marker
The BlueStar PLUS Prestained Protein Marker is a three color protein standard with 12 prestained proteins covering a wide range molecular weights from 10 – 240kDa. The Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins.
- Broad range: 10 – 240 kDa
- High performance: 12 bands with 3 colours (blue, red and green)
- Ready to use: Supplied in a loading buffer for direct loading on gels
- Easy to identify: The 25 (green) and 75 kDa (red) are reference bands
- Sharp bands
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, 2 % SDS, 0,2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Store at -20°C for 24 months.
Stable at +4°C for 3 months.
Stable at +25°C for 2 weeks.
Recommendations for Loading
- Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
- Mix thoroughly, to ensure that the solution is homogeneous.
- Load the following volumes of the ladder on SDS-polyacrylamide gel:
– 5 μl per well for mini-gels, 3 μl per well for blots;
– 10 μl per well for large gels, 6 μl per well for blots
• Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring of protein transfer onto membranes during Western blotting
• Sizing of proteins on SDS-polyacrylamide gels and Western blots
Safety data sheet (MSDS)
Which amino acids are linked to the BlueStar Plus Prestained Marker MWP04?
Amino acids involved in the dye reaction are those with amine side chain, such as Arg and Lys.
Can the tryptophane been tagged with the trihalo compounds of the biorad gels or the fluorescent dye?
We do not have information regarding the prestained dye interfering with Bio-rad gel systems or the fluorescent dye associated with it.
Does this marker contain Formaldehyde or Formamide?