BlueStar Plus Prestained Protein Marker (500 µl)

BlueStar Plus Prestained Protein Marker (500 µl)

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1 x 500 µl Protein Marker in loading buffer, sufficient for 100 mini gels or 50 large gels

Broad range:  10 – 240 kDa

 

BlueStar PLUS Prestained Protein Marker

The BlueStar PLUS Prestained Protein Marker is a three color protein standard with 12 prestained proteins covering a wide range molecular weights from 10 – 240kDa. The Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins.

Lauferhalten dse BlueStar PLUS Proteinmarkers in Tris-Glycin, Bis-Tris MOPS und Bis Tris MES Puffer

 

  • Broad range: 10 – 240 kDa
  • High performance: 12 bands with 3 colours (blue, red and green)
  • Ready to use: Supplied in a loading buffer for direct loading on gels
  • Easy to identify: The 25 (green) and 75 kDa (red) are reference bands
  • Sharp bands

Contents

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, 2 % SDS, 0,2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Storage

Store at -20°C for 24 months.
Stable at +4°C for 3 months.
Stable at +25°C for 2 weeks.

Recommendations for Loading

  1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
  2. Mix thoroughly, to ensure that the solution is homogeneous.
  3. Load the following volumes of the ladder on SDS-polyacrylamide gel:
    – 5 μl per well for mini-gels, 3 μl per well for blots;
    – 10 μl per well for large gels, 6 μl per well for blots

Applications

• Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis

• Monitoring of protein transfer onto membranes during Western blotting

• Sizing of proteins on SDS-polyacrylamide gels and Western blots

 

Downloads

Manual

MWP04 Manual

Safety data sheet (MSDS)

MWP04 MSDS

 

FAQs

Which amino acids are linked to the BlueStar Plus Prestained Marker MWP04?

Amino acids involved in the dye reaction are those with amine side chain, such as Arg and Lys.


Can the tryptophane been tagged with the trihalo compounds of the biorad gels or the fluorescent dye?

We do not have information regarding the prestained dye interfering with Bio-rad gel systems or the fluorescent dye associated with it.


Does this marker contain Formaldehyde or Formamide?

No.