BlueStar Prestained Protein Marker (25µl)

BlueStar Prestained Protein Marker (25µl)

Regular price
$13.56
Regular price
Sale price
$13.56
Unit price
per 
Availability
Sold out

Protein Marker in loading buffer

Catalogue Number: MWP03s

 

Broad range: 10 – 180 kDa

BlueStar Prestained Protein Marker

The BlueStar Prestained Protein Ladder is a three color protein standard with 10 prestained proteins covering a wide range molecular weights from 10 to 180 kDa. The BlueStar Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon or nitrocellulose) and for approximating the size of proteins.

 

Lauferhalten dse BlueStar Proteinmarkers in Tris-Glycin, Bis-Tris MOPS und Bis Tris MES Puffer

 

  • Broad range: 10 – 180 kDa
  • High performance: 10 bands with 3 colours (blue, orange and green)
  • Ready to use: Supplied in a loading buffer for direct loading on gels.
  • Reference bands: 28 kDa (green) and 75 kDa (orange)
  • Sharp bands

Storage Buffer

20 mM Disodium hydrogen phosphate pH 8.0 (at 25°C ), 2 % SDS, 0.2% Triton X-100,10 mM 2-Mercaptoethanol, 6 M Urea and 27% (v/v) Glycerol.

Storage

Stable at -20°C for 12 months.
Stable at +4°C for 3 months.

Recommendations for Loading

1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
2. Mix thoroughly, to ensure that the solution is homogeneous.
3. Load the following volumes of the ladder on SDS-polyacrylamide gel:

  • 5 μl per well for mini-gels, 3 μl per well for blots
  • 10 μl per well for large gels, 6 μl per well for blots

 

Applications

• Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis

• Monitoring of protein transfer onto membranes during Western blotting

• Sizing of proteins on SDS-polyacrylamide gels and Western blots

 

Downloads

Manual

MWP03 Manual

Safety Data Sheet (MSDS)

MWP03 MSDS

 

FAQs

Does this marker contain Formaldehyde or Formamide?

No.