{"product_id":"bmi1-mouse-monoclonal-antibody-mb9014","title":"Bmi1 Mouse Monoclonal Antibody-MB9014","description":"\u003cp\u003eBmi1 Mouse Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB9014-50, MB9014-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse IgG2a, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P35226\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC IHC FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000 \u003cbr\u003e\nIHC\/ICC: 1:100-1:200 \u003cbr\u003e\nFC: 1:100-1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Bmi-1 has been identified as a potent oncogene as it contributes to the transcriptional activation of genes implicated in early lymphoid development. Proviral activation of Bmi-1 expression corresponds to enhanced gene-specific activation of other proto-oncogenes, including c-Myc and Pim, subsequently resulting in the progression of lymphomagenesis.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Protein A affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of Bmi1 and does not cross-react with related proteins.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 37 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Bmi1 Mouse mAb at 1:1000 dilution\u003cbr\u003e\n Lane 1: 293T whole cell lysate\u003cbr\u003e\n Lane 2: Jurkat whole cell lysate\u003cbr\u003e\n Lane 3: Hela whole cell lysate\u003cbr\u003e\n Lane 4: MCF7 whole cell lysate\u003cbr\u003e\n Lane 5: HepG2 whole cell lysate\u003cbr\u003e\n Lane 6: NIH\/3T3 whole cell lysate\u003cbr\u003e\n Lane 7: PC12 whole cell lysate\u003cbr\u003e\n Lane 8: The kidney tissue lysate of Mouse\u003cbr\u003e\n Lane 9: The kidney tissue lysate of Human\u003cbr\u003e\n Lane 10: K562 whole cell lysate\u003cbr\u003e\n Lane 11: The brain tissue lysate of Human, Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bmi1 antibody. Counter stained with hematoxylin., ICC staining Bmi1 inA549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Polycomb complex protein BMI-1; Polycomb group RING finger protein 4; RING finger protein 51; BMI1; PCGF4; RNF51\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: This antibody is produced by immunizing mice with a recombinant protein corresponding to a region of Bmi1.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43044036444339,"sku":"MB9014-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43044036477107,"sku":"MB9014-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_dcb0e45c-b45e-40dc-9447-13310aaeb21b.jpg?v=1707738841","url":"https:\/\/afsbio.com\/products\/bmi1-mouse-monoclonal-antibody-mb9014","provider":"AFSBio Inc.","version":"1.0","type":"link"}