{"product_id":"bmi1-polyclonal-antibody-bs90140","title":"Bmi1 Polyclonal Antibody-BS90140","description":"\u003cp\u003eBmi1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90140-50, BS90140-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P35226(Human) EntrezGene:307151(Rat)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\/IF, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:1,000-1:2,000\u003cbr\u003e\nICC:1:50-1:200\u003cbr\u003e\nIHC:1:50-1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: In Drosophila, Polycomb (Pc-g) gene family encodes chromatin proteins that are required for the repression of homeotic loci in embryonic development. Mel-18 and Bmi-1, mammalian homologs of Drosophila Pc-g group proteins, are similarly expressed during development and implicated in the regulation of gene expression, axial skeleton development, control of proliferation and survival of haematopoietic cells. Mel-18 directly binds to DNA through a RING-finger motif and preferentially associates with juxtaposed enhancer elements on various genes, including Bcl-2, c-Myc and Hox. Mel-18 is an immediate early response gene within the c-Myc\/Cdc25 signaling cascade that exhibits tumor suppressor activity and negatively regulates cell cycle progression by blocking S phase entry. Alternatively, Bmi-1 has been identified as a potent oncogene as it contributes to the transcriptional activation of genes implicated in early lymphoid development. Proviral activation of Bmi-1 expression corresponds to enhanced gene-specific activation of other proto-oncogenes, including c-Myc and Pim, subsequently resulting in the progression of lymphomagenesis.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Bmi1 polyclonal antibody detects endogenous levels of Bmi1 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 42 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1\/1,000 dilution. \u003cbr\u003e\n Positive control: \u003cbr\u003e\n Lane 1: K562 \u003cbr\u003e\n Lane 2: PC-12, ICC staining Bmi1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: BMI1, B lymphoma Mo MLV insertion region (mouse), B lymphoma Mo MLV insertion region 1 homolog, Bmi 1, BMI1, BMI1 polycomb ring finger oncogene, BMI1, Flvi 2\/bmi 1, FLVI2\/BMI1, MGC12685, Murine leukemia viral (bmi 1) oncogene homolog, Oncogene BMI 1, PCGF 4, PCGF4, Polycomb complex protein BMI 1, Polycomb complex protein BMI-1, Polycomb group protein Bmi1, Polycomb group ring finger 4, Polycomb group RING finger protein 4, RING finger protein 51, RNF 51, RNF51,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42979764338867,"sku":"BS90140-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42979764371635,"sku":"BS90140-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_de33621e-2aaf-4f53-96f8-523a019eee20.jpg?v=1705748909","url":"https:\/\/afsbio.com\/products\/bmi1-polyclonal-antibody-bs90140","provider":"AFSBio Inc.","version":"1.0","type":"link"}