{"product_id":"brg1-monoclonal-antibody-mb66493","title":"BRG1 Monoclonal Antibody-MB66493","description":"\u003cp\u003eBRG1 Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB66493-50, MB66493-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P51532\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB (1\/500 - 1\/1000), IP (1\/10 - 1\/50)\u003c\/p\u003e\n\n\u003cp\u003eBackground: ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair . The SWI\/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin . The BRM\/BRG1 containing SWI\/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1 to regulate gene activation, cell growth, the cell cycle, and differentiation processes . BRM and BRG1 are also considered to be tumor suppressors and their expression levels are severely reduced in several cancer cell lines .\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was purified by immunogen affinity chromatography.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of BRG1 protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of BRG1 expression in Hela (A), K562 (B) whole cell lysates.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 220 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: BAF190A; BRG1; SNF2B; SNF2L4; Transcription activator BRG1; ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BAF190A; Mitotic growth and transcription activator; Protein BRG-1; Protein brahma homolog 1; SNF2-beta; SWI\/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Purified recombinant human BRG1 protein fragments expressed in E.coli.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42906362216627,"sku":"MB66493-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42906362249395,"sku":"MB66493-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_c5c17f66-ced2-4ec1-a8fd-d248378e9663.jpg?v=1702918696","url":"https:\/\/afsbio.com\/products\/brg1-monoclonal-antibody-mb66493","provider":"AFSBio Inc.","version":"1.0","type":"link"}