{"product_id":"c-ebp-β-phospho-t235-188-polyclonal-antibody-bs4029","title":"C\/EBP-β (phospho-T235\/188) Polyclonal Antibody-BS4029","description":"\u003cp\u003eC\/EBP-β (phospho-T235\/188) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS4029-50, BS4029-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P17676\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eC\/EBP-β (phospho-T235\/188) Polyclonal Antibody-BS4029\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: C\/EBP β is a member of the C\/EBP transcription factor family. The C\/EBP β gene encodes several isoforms, which have truncated transcription activation domains by the alternative translational initiation at multipe AUG start sites. Initiation of translation at the in-frame AUGs forms four C\/EBP β isoforms. C\/EBP β is also known as interleukin 6-dependent DNA-binding protein (IL6DBP), liver activator protein(LAP) or liver-enriched transcriptional activator protein transcription factor 5 (TCF5). C\/EBP β contributes to the regulation of the acute phase response in hepatocytes. Stat3 has an important function in IL-6-mediated transcription of the C\/EBP β gene that has direct implication for acute phase response in liver cells.The 35 kDa C\/EBP β form requires phosphorylation for its DNA binding ability, and increased binding of C\/EBP β isoforms during acute-phase reaction occurs through its upregulation and structural modification.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: p-C\/EBP-β (T235\/188) polyclonal antibody detects endogenous levels of human LAP only when phosphorylated at Thr235, mouse and rat LAP only when phosphorylated at Thr188, and LIP only when phosphorylated at Thr37.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 38 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of C\/EBP-β (phospho-T235\/188) pAb at 1:500 dilution\u003cbr\u003e\n Lane 1: MCF-7 treated with insulin (500ng\/ml,5min) whole cell lysate (40ug) \u003cbr\u003e\n Lane 2: MCF-7 treated with insulin (500ng\/ml,0min) whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p-C\/EBP-β (T235\/188) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: CCAAT\/enhancer-binding protein beta; C\/EBP β; C\/EBPβ; C\/EBP beta; Liver activator protein; LAP; Liver-enriched inhibitory protein; LIP; Nuclear factor NF-IL6; Transcription factor 5; TCF-5; CEBPB; TCF5; ORF Names: PP9092\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic phosphopeptide derived from human C\/EBP-β around the phosphorylation site of Threonine 235.\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42957294600371,"sku":"BS4029-50","price":276.71,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42957294633139,"sku":"BS4029-100","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_70eba121-149b-45a6-ae85-0f607202c342.jpg?v=1705144483","url":"https:\/\/afsbio.com\/products\/c-ebp-%ce%b2-phospho-t235-188-polyclonal-antibody-bs4029","provider":"AFSBio Inc.","version":"1.0","type":"link"}