{"product_id":"c-fos-polyclonal-antibody-bs6433","title":"c-Fos Polyclonal Antibody-BS6433","description":"\u003cp\u003ec-Fos Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50 µl, 100 µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS6433-50, BS6433-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P01100\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003ec-Fos Polyclonal Antibody-BS6433\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP,\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000  |  IP,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Polyclonal Antibodies\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 55-60KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of HeLa cells, using c-Fos antibody at 1:1000 dilution.HeLa cells were treated by PMA\/TPA at 37℃ for 15 minutes after serum-starvation overnight.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 1s., Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug c-Fos antibody. Western blot was performed from the immunoprecipitate using c-Fos antibody at a dilution of 1:1000.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: FOS; AP-1; C-FOS; p55; c-Fos\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human c-Fos(NP_005243.1).\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42962829705395,"sku":"BS6433-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42962829738163,"sku":"BS6433-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_4ed21ed9-eaec-46b8-9db0-d51fff4801db.jpg?v=1705319200","url":"https:\/\/afsbio.com\/products\/c-fos-polyclonal-antibody-bs6433","provider":"AFSBio Inc.","version":"1.0","type":"link"}