{"product_id":"caffeine-elisa-kit-elk0683","title":"Caffeine ELISA Kit - ELK0683","description":"\u003cp\u003eCaffeine ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK0683-48, ELK0683-96, ELK0683-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: Caffeine\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.25 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 30 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.47-30 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Serum, plasma, other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Caffeine. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Caffeine. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caffeine in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43211520049331,"sku":"ELK0683-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43211520082099,"sku":"ELK0683-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200640442547,"sku":"ELK0683-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_9430443b-f3d0-4c3b-9fed-86e99185ea82.png?v=1767060667","url":"https:\/\/afsbio.com\/products\/caffeine-elisa-kit-elk0683","provider":"AFSBio Inc.","version":"1.0","type":"link"}