{"product_id":"calnexin-phospho-s583-polyclonal-antibody-bs4262","title":"Calnexin (phospho-S583) Polyclonal Antibody-BS4262","description":"\u003cp\u003eCalnexin (phospho-S583) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS4262-50, BS4262-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P27824\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:10000~1:50000\u003cbr\u003e\nIHC: 1:50~1:1000\u003cbr\u003e\nIP: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Calnexin and Calregulin (also called Calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum—Calnexin to the membrane and Calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor Calregulin contains the calcium-binding “E-F hand” motif found in calmodulins. Calnexin and Calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: p-Calnexin (S583) polyclonal antibody detects endogenous levels of Calnexin protein only when phosphorylated at Ser583\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 90 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Calnexin (phospho-S583) pAb at 1:10000 to 1:50000 dilution\u003cbr\u003e\n Lane 1: HEK293T whole cell lysate (40ug)\u003cbr\u003e\n Lane 2: SGC7901 whole cell lysate (40ug)\u003cbr\u003e\n Lane 3: A375 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p-Calnexin (S583) pAb in paraffin-embedded human breast carcinoma tissue at 1:100., Immunoprecipitation of L02 whole cell lysate using Calnexin (phospho-S583) pAb (Sepharose Bead Conjugate) #BD0047(Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (Lane 4 and Lane 5). Lane 1 is 20% input. The western blot was probed using Calnexin (phospho-S583) pAb #BS4262. “↑”（supernatant); “↓”（deposition）\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Calnexin; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90; CANX\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic phosphopeptide derived from human Calnexin around the phosphorylation site of Serine 583.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42993819254963,"sku":"BS4262-50","price":276.71,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42993819287731,"sku":"BS4262-100","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_04a8c73f-415d-4cba-bb60-44033f690376.jpg?v=1706183924","url":"https:\/\/afsbio.com\/products\/calnexin-phospho-s583-polyclonal-antibody-bs4262","provider":"AFSBio Inc.","version":"1.0","type":"link"}