CALIXAR’s CALX8 reagent is an ionic calixarene based detergent/surfactant with three polar carboxylic groups and an octyl chain.
5,11,17-tris[(carboxy)methyl]-25-monooctyloxy-26,27,28-trihydroxycalix[4]arene
Compound name: CALX8
Catalogue number: CALX8_50MG, CALX8_100MG, CALX8_250MG
Molec. Formula: C42H43Na3O10
CAS: nd
MW: 776.7 g/mol
pKa: na
Percent Composition: C, 64.94; H, 5.58; N, 8.88; O, 20.60
Physical state: Beige powder
Purity (HPLC, 214nm): nd
Retention time (RP18 HPLC): nd
CMC: 2.2 mM
Exact mass: 776.2549
Stability: Store in <-20°C freezer for up to one year
Solubility Structure: Soluble in methanol, DMSO and water
CALIXAR employs a unique & custom-made procedure
The Calixarene platform and carboxylic groups help to stabilize native membrane proteins and the alkyl chain serves to extract native membrane proteins at the same time.
CALIXAR’s CALX8 is in powder form and is used in an aqueous solution or buffer. CALX8 can be combined with biological materials (such as biological membranes).
Clients are able to use CALX8 to assist in the development of lipid detergents, mixed micelles, and protein detergent micelles. CALX8 enables clients to extract, solubilize and stabilize native and functional membrane proteins in a solution.
CALX8 is very mild detergent to secure, solubilize and preserve native membrane proteins. It is also not sensitive to ionic strength or pH variations which is ideal for extraction, to solubilization and to stabilization of particular membrane proteins.
CALIXAR’s CALX8 is non-ionic and consequently is not susceptible to ionic strength or pH variations and is adapted to extract, to solubilize or to stabilize precise membrane proteins.
CALIXAR’s CALX8 extracts high-quality membrane proteins that are utilized for pharmaceutical research, discovery and, structural studies. Our CALX8 is used by biotechnology companies, as well as for academic teams (biochemists, structural biologists, pharmacologists, virologists) that are involved in the life science fields.
- Antibodies (including nanobodies, scaffold proteins, aptamers)
- Small molecules
- 3D Structures (cryoEM, XRay crystallography, NMR, SANS, SAXS)
- Drug discovery (Screening: HTS, FBDD, SBDD; Hit and lead validation)
- Antibody discovery (Immunization and display technologies)
- Clinical stage
ANALYTICAL BIOCHEMISTRY
Novel systematic detergent screening method for membrane proteins solubilization
Desuzinges Mandon E. et al. 2017