{"product_id":"camkiα-phospho-thr177-rabbit-polyclonal-antibody-es7812","title":"CaMKIα (phospho Thr177) Rabbit Polyclonal Antibody - ES7812","description":"\u003cp\u003eCaMKIα (phospho Thr177) Rabbit Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES7812-50, ES7812-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: Calcium\/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium\/calmodulin directly activates calcium\/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium\/calmodulin-dependent protein kinase I kinase. [provided by RefSeq, Jul 2008],\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: CAMK1, Calcium\/calmodulin-dependent protein kinase type 1, CaM kinase I, CaM-KI, CaM kinase I alpha, CaMKI-alpha\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human CaMK1-alpha around the phosphorylation site of Thr177. AA range:143-192\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eTarget: CaMKIα\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phospho-CaMKIα (T177) Polyclonal Antibody detects endogenous levels of CaMKIα protein only when phosphorylated at T177.\u003c\/p\u003e\n\n\u003cp\u003eDilution: Western Blot: 1\/500 - 1\/2000. Immunohistochemistry: 1\/100 - 1\/300. Immunofluorescence: 1\/200 - 1\/1000. ELISA: 1\/5000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml,\u003c\/p\u003e\n\n\u003cp\u003eFormlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eExpression: Widely expressed, Expressed in cells of the zona glomerulosa of the adrenal cortex,\u003c\/p\u003e\n\n\u003cp\u003eObserved Band (KD): 41kD\u003c\/p\u003e\n\n\u003cp\u003eFields: Calcium signaling pathway, Oxytocin signaling pathway, Aldosterone synthesis and secretion, Glioma\u003c\/p\u003e\n\n\u003cp\u003eFunction: Catalytic Activity: ATP + a protein = ADP + a phosphoprotein\u003cbr\u003e\nDomain: The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate\u003cbr\u003e\nEnzyme Regulation: Activated by Ca(2+)\/calmodulin. Binding of calmodulin results in a conformational change that generates functional binding sites for both, substrate and ATP, and thus releaves intrasteric autoinhibition. Must be phosphorylated to be maximally active. Phosphorylated by CAMKK1 or CAMKK2\u003cbr\u003e\nFunction: Calcium\/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes like transcriptional regulation, hormone production, translational regulation, regulation of actin filament organization and neurite outgrowth. Involved in calcium-dependent activation of the ERK pathway (By si\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: Cytoplasm Nucleus Predominantly cytoplasmic\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eGene Name: CAMK1\u003c\/p\u003e\n\n\u003cp\u003eProtein Name: Calcium\/calmodulin-dependent protein kinase type 1\u003c\/p\u003e\n\n\u003cp\u003eMouse Gene ID: 52163\u003c\/p\u003e\n\n\u003cp\u003eMouse SwissProt Number: Q91YS8\u003c\/p\u003e\n\n\u003cp\u003eRat Gene ID: 171503\u003c\/p\u003e\n\n\u003cp\u003eRat SwissProt Number: Q63450\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":46313757573299,"sku":"ES7812-50","price":186.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":46313757606067,"sku":"ES7812-100","price":372.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/elk_antibodies_d75295e2-ffc1-4e0f-a3e7-87715c8983a9.jpg?v=1793190507","url":"https:\/\/afsbio.com\/products\/camki%ce%b1-phospho-thr177-rabbit-polyclonal-antibody-es7812","provider":"AFSBio Inc.","version":"1.0","type":"link"}