{"product_id":"camp-cyclic-adenosine-monophosphate-elisa-kit-elk8116","title":"cAMP (Cyclic Adenosine Monophosphate) ELISA Kit - ELK8116","description":"\u003cp\u003ecAMP (Cyclic Adenosine Monophosphate) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK8116-48, ELK8116-96, ELK8116-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adenosine Cyclophosphate\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 92.2 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 20000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 312.5-20000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction; Metabolic pathway;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with cAMP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to cAMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of cAMP in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212359172275,"sku":"ELK8116-48","price":518.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212359205043,"sku":"ELK8116-96","price":739.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200790847667,"sku":"ELK8116-5x96","price":3141.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_721a6c24-3861-4d1e-818f-38f1acb283a2.png?v=1767063885","url":"https:\/\/afsbio.com\/products\/camp-cyclic-adenosine-monophosphate-elisa-kit-elk8116","provider":"AFSBio Inc.","version":"1.0","type":"link"}