{"product_id":"car-polyclonal-antibody-gcp44","title":"CAR Polyclonal Antibody-GCP44","description":"\u003cp\u003eCAR Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: GCP44-50, GCP44-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P78310\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human,Pig\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000~1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: The coxsackie virus and adenovirus receptor (CXADR, CAR) is a highly conserved, single-transmembrane glycoprotein and the primary receptor to mediate cellular attachment and infection of coxsackie B viruses and most adenoviruses. The CAR protein contains a pair of Ig-like domains within the amino-terminal extracellular domain and a carboxyl-terminal PDZ motif. Research studies indicate that CAR is a tight junction protein that associates with the ZO-1 scaffold protein and promotes both cell adhesion and restriction of solute and ion movement between cells. Endogenous CAR is targeted to the basolateral plasma membrane by a tyrosine-based basolateral sorting signal and clathrin adaptors AP-1A and AP-1B. CAR binds junctional adhesion molecule L (JAML) on epithelial cells and neutrophils where it activates PI3K and downstream MAPK kinases to stimulate epithelial γδ T cell proliferation and increase production of TNFα and keratinocyte growth factor. As a result, the CAR protein plays a potentially critical role in adenoviral gene therapy, immunity, wound repair, inflammation, and cancer therapy. Additional studies demonstrate that CAR is essential in regulating squamous carcinoma cell growth.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: CAR polyclonal antibody detects endogenous levels of CAR protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of CAR polyclonal antibody at 1:1000 dilution\u003cbr\u003e\nLane1:EC9706 whole cell lysate(40ug)\u003cbr\u003e\nLane2:The Lung tissue lysate of Pig(40ug)\u003cbr\u003e\nLane3:The Heart tissue lysate of Pig(40ug)\u003cbr\u003e\nLane4:LOVO whole cell lysate(40ug)\u003cbr\u003e\nLane5:HepG2 whole cell lysate(20ug)\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 50 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Coxsackievirus and adenovirus receptor; CAR; hCAR; CVB3-binding protein; Coxsackievirus B-adenovirus receptor; HCVADR; CXADR; CAR\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to Human CAR.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42908756541619,"sku":"GCP44-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42908756574387,"sku":"GCP44-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_abefc53e-6540-473c-9e4b-c8ed58135951.jpg?v=1703006700","url":"https:\/\/afsbio.com\/products\/car-polyclonal-antibody-gcp44","provider":"AFSBio Inc.","version":"1.0","type":"link"}