{"product_id":"caspase-3-polyclonal-antibody-bs90181","title":"Caspase 3 Polyclonal Antibody-BS90181","description":"\u003cp\u003eCaspase 3 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90181-50, BS90181-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P42574(Human)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB:1:1,000-1:2,000\u003cbr\u003e\nIHC:1:50-1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Caspase 3 polyclonal antibody detects endogenous levels of Caspase 3 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 35 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of active+pro Caspase 3 on Jurkat cells lysates using anti-active+pro Caspase 3 antibody at 1\/1,000 dilution., Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-active+pro Caspase 3 antibody. Counter stained with hematoxylin.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: CASP-3, CPP-32, SCA-1, CASP3, Apopain, CASP 3, CASP-3, CASP3, CASP3, Caspase 3, Caspase-3 subunit p12, CPP 32, CPP-32, CPP32B, Cysteine protease CPP32, PARP cleavage protease, Protein Yama, SCA-1, SCA1, SREBP cleavage activity 1, Yama,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42979767156915,"sku":"BS90181-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42979767189683,"sku":"BS90181-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_b6ec7335-246d-482e-9785-8b87cc4d4013.jpg?v=1705748992","url":"https:\/\/afsbio.com\/products\/caspase-3-polyclonal-antibody-bs90181","provider":"AFSBio Inc.","version":"1.0","type":"link"}