{"product_id":"catenin-β-q27-polyclonal-antibody-bs3603","title":"Catenin-β (Q27) Polyclonal Antibody-BS3603","description":"\u003cp\u003eCatenin-β (Q27) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS3603-50, BS3603-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P35222\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003cbr\u003e\nIF: 1:50~1:200\u003cbr\u003e\nIP: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: β-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Catenin-β (Q27) polyclonal antibody detects endogenous levels of Catenin-β protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 92 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Catenin-β (Q27) pAb at 1:500 dilution\u003cbr\u003e\n Lane 1: C6 whole cell lysate (40ug)\u003cbr\u003e\n Lane 2: 3T3-L1 whole cell lysate (40ug)\u003cbr\u003e\n Lane 3: Hela whole cell lysate (40ug)\u003cbr\u003e\n Lane 4: HEK293T whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Catenin-β (Q27) pAb in paraffin-embedded human breast carcinoma tissue at 1:100., Immunoprecipitation of HEK293T cell lysates using Catenin-β (Q27) pAb (Sepharose Bead Conjugate)#BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using Catenin-β (Q27) pAb.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Catenin β; Cateninβ; Catenin beta-1; Beta-catenin; CTNNB1; CTNNB; ORF Names: OK\/SW-cl.35; PRO2286\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human Catenin-β.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43044051484851,"sku":"BS3603-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43044051517619,"sku":"BS3603-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_7309d3d0-edf3-44da-b38e-7b978d187f01.jpg?v=1707739078","url":"https:\/\/afsbio.com\/products\/catenin-%ce%b2-q27-polyclonal-antibody-bs3603","provider":"AFSBio Inc.","version":"1.0","type":"link"}