{"product_id":"cattle-ap-aprotinin-elisa-kit-elk6113","title":"Cattle AP (Aprotinin) ELISA Kit - ELK6113","description":"\u003cp\u003eCattle AP (Aprotinin) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK6113-48, ELK6113-96, ELK6113-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: BPTI; Trasylol; Pancreatic Trypsin Inhibitor; Basic protease inhibitor\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.55 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 100 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 1.57-100 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Enzyme and Kinase; Cardiovascular biology; Human gene deletion; Hepatology;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle AP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle AP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle AP in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43211853725875,"sku":"ELK6113-48","price":576.8,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43211853758643,"sku":"ELK6113-96","price":823.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200750575795,"sku":"ELK6113-5x96","price":3498.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_4ce7342c-d738-4d57-8395-1475ebbf3e3f.png?v=1767063110","url":"https:\/\/afsbio.com\/products\/cattle-ap-aprotinin-elisa-kit-elk6113","provider":"AFSBio Inc.","version":"1.0","type":"link"}