{"product_id":"cattle-bhb-beta-hydroxybutyric-acid-elisa-kit-elk8618","title":"Cattle bHB (Beta-Hydroxybutyric Acid) ELISA Kit - ELK8618","description":"\u003cp\u003eCattle bHB (Beta-Hydroxybutyric Acid) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK8618-48, ELK8618-96, ELK8618-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: OHB; Beta-Hydroxybutyrate; 3-Hydroxybutyric Acid\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.46 µg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 100 µg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 1.57-100 µg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction; Metabolic pathway; Endocrinology;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle bHB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle bHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle bHB in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212643205299,"sku":"ELK8618-48","price":371.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212643238067,"sku":"ELK8618-96","price":526.4,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200800841907,"sku":"ELK8618-5x96","price":2237.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_361ae6bf-726c-4076-8d6a-a7bd0918d442.png?v=1767064280","url":"https:\/\/afsbio.com\/products\/cattle-bhb-beta-hydroxybutyric-acid-elisa-kit-elk8618","provider":"AFSBio Inc.","version":"1.0","type":"link"}