{"product_id":"cattle-casp8-caspase-8-elisa-kit-elk9510","title":"Cattle CASP8 (Caspase 8) ELISA Kit - ELK9510","description":"\u003cp\u003eCattle CASP8 (Caspase 8) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK9510-48, ELK9510-96, ELK9510-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE\/ced-3-like protease; MORT1-associated ced-3 homolog\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.112 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 20 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.32-20 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Tissue homogenates, cell lysates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction; Enzyme and Kinase; Apoptosis; Tumor immunity; Neuroscience;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle CASP8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle CASP8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle CASP8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle CASP8 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212820447411,"sku":"ELK9510-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212820480179,"sku":"ELK9510-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200825188531,"sku":"ELK9510-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_d7d65306-ba1f-4232-90f7-866f174de925.png?v=1767064984","url":"https:\/\/afsbio.com\/products\/cattle-casp8-caspase-8-elisa-kit-elk9510","provider":"AFSBio Inc.","version":"1.0","type":"link"}