{"product_id":"cattle-e2-estradiol-elisa-kit-elk10163","title":"Cattle E2 (Estradiol) ELISA Kit - ELK10163","description":"\u003cp\u003eCattle E2 (Estradiol) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK10163-48, ELK10163-96, ELK10163-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: 17B-Estradiol; Oestradiol; Beta-Estradiol\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 14.42 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 3000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 46.88-3000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Endocrinology; Reproductive science; Hormone metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle E2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle E2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle E2 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45200657252531,"sku":"ELK10163-48","price":371.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45200657285299,"sku":"ELK10163-96","price":526.4,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200657318067,"sku":"ELK10163-5x96","price":2237.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_76e0f5b4-34f7-453a-8a66-2c2676f321a8.png?v=1767061102","url":"https:\/\/afsbio.com\/products\/cattle-e2-estradiol-elisa-kit-elk10163","provider":"AFSBio Inc.","version":"1.0","type":"link"}