{"product_id":"cattle-f2-coagulation-factor-ii-elisa-kit-elk8258","title":"Cattle F2 (Coagulation Factor II) ELISA Kit - ELK8258","description":"\u003cp\u003eCattle F2 (Coagulation Factor II) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK8258-48, ELK8258-96, ELK8258-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: FII; TM; PT; Thrombin; Prothrombin; Pro-Thrombin; Activation peptide fragment 1; Activation peptide fragment 2\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.276 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 40 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.63-40 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction; Hematology;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle F2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle F2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle F2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle F2 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: P00735\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43213912506547,"sku":"ELK8258-48","price":576.8,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43213912539315,"sku":"ELK8258-96","price":823.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45201409835187,"sku":"ELK8258-5x96","price":3498.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_5121c1ce-5d82-4a34-aa35-0258f06f49ff.png?v=1767096389","url":"https:\/\/afsbio.com\/products\/cattle-f2-coagulation-factor-ii-elisa-kit-elk8258","provider":"AFSBio Inc.","version":"1.0","type":"link"}