{"product_id":"cattle-hsp-70-hspa9-heatshock-protein-70-elisa-kit-elk5258","title":"Cattle HSP-70\/HSPA9 (HeatShock Protein 70) ELISA Kit - ELK5258","description":"\u003cp\u003eCattle HSP-70\/HSPA9 (HeatShock Protein 70) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK5258-48, ELK5258-96, ELK5258-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: DnaK; 70 Kilodalton Heat Shock Proteins; Heat shock 70 kDa protein 1; 2; heat shock 70kDa protein 1A; HSP70; HSP70 1; HSP70 1; HSP70 2; HSP70 1A; HSP70.1; HSP70.2; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B; HSP70; Heat Shock Protein 70\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 1.22 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 200 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 3.13-200 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Tumor immunity; Infection immunity; Developmental science;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle HSP-70\/HSPA9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle HSP-70\/HSPA9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle HSP-70\/HSPA9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle HSP-70\/HSPA9 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: Q3ZCH0\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43213579714739,"sku":"ELK5258-48","price":576.8,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43213579747507,"sku":"ELK5258-96","price":823.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45201336500403,"sku":"ELK5258-5x96","price":3498.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_4566f017-9ae6-41f6-a6d8-ae7bc9384cdb.png?v=1767093102","url":"https:\/\/afsbio.com\/products\/cattle-hsp-70-hspa9-heatshock-protein-70-elisa-kit-elk5258","provider":"AFSBio Inc.","version":"1.0","type":"link"}