{"product_id":"cattle-hspd1-heat-shock-60kd-protein-1-chaperonin-elisa-kit-elk0295","title":"Cattle HSPD1 (Heat Shock 60kD Protein 1, Chaperonin) ELISA Kit - ELK0295","description":"\u003cp\u003eCattle HSPD1 (Heat Shock 60kD Protein 1, Chaperonin) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK0295-48, ELK0295-96, ELK0295-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: HSP60; GROEL; CPN60; HuCHA60; Heat Shock Protein 60; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.72 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 100 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 1.57-100 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction; Tumor immunity;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle HSPD1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle HSPD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle HSPD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle HSPD1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: P31081\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212879134899,"sku":"ELK0295-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212879167667,"sku":"ELK0295-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200859726003,"sku":"ELK0295-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_c51ae499-c302-43a5-bb86-c57d2dcdc620.png?v=1767065710","url":"https:\/\/afsbio.com\/products\/cattle-hspd1-heat-shock-60kd-protein-1-chaperonin-elisa-kit-elk0295","provider":"AFSBio Inc.","version":"1.0","type":"link"}