{"product_id":"cattle-vb3-vitamin-b3-elisa-kit-elk11316","title":"Cattle VB3 (Vitamin B3) ELISA Kit - ELK11316","description":"\u003cp\u003eCattle VB3 (Vitamin B3) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK11316-48, ELK11316-96, ELK11316-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: VitB3,VB3\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.93 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 200 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 3.13-200 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Metabolic pathway; Reproductive science; Nutrition metabolism; Bone metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle VB3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle VB3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle VB3 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45200705749171,"sku":"ELK11316-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45200705781939,"sku":"ELK11316-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200705814707,"sku":"ELK11316-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_1b98cfef-a7d0-4150-9a51-8443908fd1ed.png?v=1767061918","url":"https:\/\/afsbio.com\/products\/cattle-vb3-vitamin-b3-elisa-kit-elk11316","provider":"AFSBio Inc.","version":"1.0","type":"link"}