{"product_id":"cbp80-r5-polyclonal-antibody-bs2040","title":"CBP80 (R5) Polyclonal Antibody-BS2040","description":"\u003cp\u003eCBP80 (R5) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS2040-50, BS2040-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q09161\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e \n\n\u003cp\u003eBackground: In eukaryotes, the majority of mRNAs have an m (7)G cap, which is added cotranscriptionally and plays a critical role in many aspects of mRNA metabolism. The effect of the cap on translation is mediated by the initiation factor eIF-4F, whereas the effect on pre-mRNA splicing involves a nuclear complex(CBC). CBC consists of two cap binding proteins CBP20 and CBP80, which mediate the stimulatory functions of the cap in pre-mRNA splicing, 3' end formation and U snRNA export. The genes CBC1 and CBC2 encode CBP80 and CBP20, respectively. CBP80 comprises three domains, each containing a MIF4G domain. CBP20 has an RNAP fold and associates with the second and third domains of CBP80.\u003c\/p\u003e \n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: CBP80 (R5) polyclonal antibody detects endogenous levels of CBP80 protein.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of CBP80 (R5) pAb at 1:500 dilution\u003cbr\u003e\nLane 1: K562 whole cell lysate (10ug)\u003cbr\u003e\nLane 2: Hela whole cell lysate (20ug)\u003cbr\u003e\nLane 3: The Brain tissue lysate of Mouse (40ug)\u003cbr\u003e\nLane 4: The Brain tissue lysate of Rat (40ug), Immunohistochemistry (IHC) analyzes of CBP80 (R5) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: ~ 88 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Nuclear cap-binding protein subunit 1; 80 kDa nuclear cap-binding protein; CBP80; NCBP 80 kDa subunit; NCBP1; CBP80; NCBP\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to the N-terminus of Human CBP80.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42953219244211,"sku":"BS2040-50","price":254.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42953219276979,"sku":"BS2040-100","price":442.75,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_3e950451-8604-48c8-b41d-450e64d856cc.jpg?v=1705057004","url":"https:\/\/afsbio.com\/products\/cbp80-r5-polyclonal-antibody-bs2040","provider":"AFSBio Inc.","version":"1.0","type":"link"}