{"product_id":"cd100-rabbit-monoclonal-antibody-mb66385","title":"CD100 Rabbit Monoclonal Antibody-MB66385","description":"\u003cp\u003eCD100 Rabbit Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB66385-50, MB66385-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q92854\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB (1\/500 - 1\/1000), IF\/ICC (1\/50 - 1\/100)\u003c\/p\u003e\n\n\u003cp\u003eBackground: Semaphorin-4D\/CD100 (Sema4D) is a disulfide-linked homodimeric type 1 transmembrane glycoprotein belonging to the class IV family of membrane bound semaphorins. The extracellular domain of Sema4D contains a cysteine-rich semaphorin-like domain, an Ig-like domain, and a PSI domain . Research studies have suggested that the cytoplasmic domain has a signaling function as it is phosphorylated on serine residues . Initial studies involving Sema4D revealed that it was implicated in axon guidance within the central nervous system through binding its high affinity receptor, plexin-B1 . Sema4D function has also been extensively characterized in the immune system and is the first semaphorin found to be expressed on the surface of many types of immune cells . In the immune system, CD72 serves as a low-affinity receptor for Sema4D  and research studies have shown that Sema4D not only regulates T-cell activation  but is also involved in the regulation of B-cell survival and differentiation . Many of the physiologic effects of Sema4D in the immune system are regulated by a soluble extracellular domain-containing fragment generated through proteolytic cleavage .Sema4D has also been implicated in oncogenesis as research studies have demonstrated overexpression in multiple types of solid tumors . The role of Sema4D in oncogenesis, in part, has been linked to its ability to promote tumor angiogenesis , cell invasion , and immunosuppression through enhancement of myeloid derived suppressor cell function .\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was purified by immunogen affinity chromatography.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of CD100 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 120 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of CD100 expression in Jurkat (A) whole cell lysates., Immunofluorescent analysis of CD100 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: C9orf164; CD100; SEMAJ; Semaphorin-4D; A8; BB18; GR3; CD100\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide of human Semaphorin 4D\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42987096441011,"sku":"MB66385-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42987096473779,"sku":"MB66385-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_226f233a-8828-4af6-a076-898837b18689.jpg?v=1706009136","url":"https:\/\/afsbio.com\/products\/cd100-rabbit-monoclonal-antibody-mb66385","provider":"AFSBio Inc.","version":"1.0","type":"link"}