{"product_id":"cd111-nectin1-recombinant-protein-ncp0247","title":"CD111\/NECTIN1 Recombinant Protein - NCP0247","description":"\u003cp\u003eCD111\/NECTIN1 Recombinant Protein\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: NCP0247-500, NCP0247-1000\u003c\/p\u003e\n\n\u003cp\u003eSizes: 500ug, 1mg\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q15223\u003c\/p\u003e\n\n\u003cp\u003eHost: E.coli\u003c\/p\u003e\n\n\u003cp\u003eTag: His-tag\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: CAGGTTGTTCAGGTGAATGATAGTATGTATGGCTTCATTGGTACCGATGTTGTTCTGCATTGCAGCTTCGCAAATCCGCTGCCGAGTGTGAAAATTACCCAGGTGACCTGGCAGAAAAGCACCAATGGCAGCAAACAGAATGTTGCAATCTATAATCCGAGTATGGGCGTGAGTGTGCTGGCCCCGTATCGTGAACGTGTGGAATTCCTGCGTCCGAGCTTCACCGATGGTACCATTCGTCTGAGTCGCCTGGAACTGGAAGATGAAGGTGTGTATATCTGTGAATTCGCAACCTTCCCGACCGGTAATCGTGAAAGCCAGCTGAATCTGACCGTGATGGCAAAACCGACCAATTGGATTGAAGGCACCCAGGCAGTGCTGCGTGCAAAAAAAGGCCAGGATGATAAAGTTCTGGTTGCCACCTGTACCAGTGCAAATGGCAAACCGCCGAGTGTGGTGAGCTGGGAAACCAGACTGAAAGGTGAAGCCGAATATCAGGAAATTCGCAATCCGAATGGCACCGTGACCGTGATTAGCCGCTATCGCCTGGTTCCGAGTCGCGAAGCCCATCAGCAGAGCCTGGCATGCATTGTGAATTATCACATGGATCGCTTCAAAGAAAGCCTGACCCTGAATGTGCAGTATGAACCGGAAGTTACCATTGAAGGCTTCGATGGCAATTGGTATCTGCAGCGCATGGATGTGAAACTGACCTGCAAAGCCGATGCAAATCCGCCGGCAACCGAATATCATTGGACCACCCTGAATGGTAGCCTGCCGAAAGGCGTGGAAGCACAGAATCGCACCCTGTTCTTCAAAGGTCCGATTAATTATAGCCTGGCCGGCACCTATATCTGTGAGGCAACCAATCCGATTGGTACCCGCAGTGGTCAGGTGGAAGTGAATATTACCGAATTCCCGTATACCCCGAGTCCGCCGGAACATGGCCGTCGTGCAGGTCCGGTTCCGACCGCA\u003c\/p\u003e\n\n\u003cp\u003eRestriction Sites: NdeI-XhoI\u003c\/p\u003e\n\n\u003cp\u003eBackground: Nectin is a Ca2+-independent homophilic cell adhesion molecule that belongs to the immunoglobulin superfamily. Human Nectin is identical to the poliovirus receptor-related protein (PRR) and is identified to be the α herpesvirus entry mediator. Nectin constitutes a family consisting of at least Nectin 1, 2 and 3. Nectin 2 and 3 are ubiquitously expressed, whereas Nectin 1 is abundantly expressed in the brain. Nectin 1 exists as Nectin 1α and 1β\/HIgR, produced by alternative splicing. The cytoplasmic regions of Nectin 1α, but not Nectin 1β\/ HIgR, have a C-terminal conserved motif (E\/A-X-Y-V). This motif interacts with the PDZ domain of the F-Actin-binding protein, afadin, through which it is linked to the Actin cytoskeleton. Nectin 1, also designated HveC\/ PRR1, allows the entry of herpes simplex virus type 1 (HSV-1) and HSV-2 into mammalian\u003cbr\u003e\ncells. The interaction of virus envelope glycoprotein D (gD) with Nectin 1 is an essential step in the process leading to membrane fusion; the gD binding site is located at the first Ig-like domain of Nectin 1. Both the transinteraction of Nectin and the interaction of Nectin with afadin are necessary for their co-localization with E-cadherin and catenins at adherens junctions.\u003c\/p\u003e\n\n\u003cp\u003eSoluble: PBS, 4M Urea, PH7.4\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is \u0026gt; 85% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eExpression Vector: pet-22b(+)\u003c\/p\u003e\n\n\u003cp\u003eBioWorld Molecular Weight: ~36kDa\u003c\/p\u003e\n\n\u003cp\u003eNotes: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"500ug","offer_id":42887062585523,"sku":"NCP0247-500","price":1050.83,"currency_code":"CAD","in_stock":true},{"title":"1mg","offer_id":42887062618291,"sku":"NCP0247-1000","price":1857.89,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_02562b22-1e92-41b6-9ed7-eb2cbededa11.jpg?v=1702317316","url":"https:\/\/afsbio.com\/products\/cd111-nectin1-recombinant-protein-ncp0247","provider":"AFSBio Inc.","version":"1.0","type":"link"}