{"product_id":"cd133-polyclonal-antibody-ncp0261p","title":"CD133 Polyclonal Antibody-NCP0261P","description":"\u003cp\u003eCD133 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: NCP0261P-50, NCP0261P-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O43490\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000~1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells. Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicted molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis. Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells. A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: CD133 polyclonal antibody detects endogenous levels of CD133 protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of CD133 polyclonal antibody at 1:1000 dilution\u003cbr\u003e\nLane1:L02 whole cell lysate(30ug)\u003cbr\u003e\nLane2:HepG2 whole cell lysate(30ug)\u003cbr\u003e\nLane3:Panc1 whole cell lysate(30ug)\u003cbr\u003e\nLane4:H1792 whole cell lysate(30ug)\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 110 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Prominin-1; Antigen AC133; Prominin-like protein 1; CD133; PROM1; PROML1; MSTP061\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant protein of human CD133.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42908704768179,"sku":"NCP0261P-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42908704800947,"sku":"NCP0261P-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_2c6a38e5-0237-4719-8ee7-4dcafbf833a0.jpg?v=1703006044","url":"https:\/\/afsbio.com\/products\/cd133-polyclonal-antibody-ncp0261p","provider":"AFSBio Inc.","version":"1.0","type":"link"}