{"product_id":"cd147-polyclonal-antibody-ncp0177p","title":"CD147 Polyclonal Antibody-NCP0177P","description":"\u003cp\u003eCD147 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: NCP0177P-50, NCP0177P-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P35613\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:2000~1:5000\u003cbr\u003e\nIP  1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Basigin (EMMPRIN, CD147) is a type I integral membrane receptor protein belonging to the immunoglobulin superfamily. Basigin is a glycosylated protein with four known isoforms, of which isoform 2 is the most abundantly expressed. Multiple functions have been ascribed to Basigin; foremost among these is stimulating the secretion of extracellular matrix metalloproteinases by adjacent fibroblasts, a function which has been implicated in promoting tumor progression. Research studies have shown that Basigin is overexpressed by many tumor cells, and its expression level may correlate with tumor malignancy. A recent study identified the BASIGIN gene as a regulatory target of Slug, suggesting a role for Basigin in the process of epithelial-mesenchymal transition. Basigin has also been identified as a marker for a subset of highly suppressive regulatory T cells, and as an obligate receptor for the malarial parasite Plasmodium falciparum on human erythrocytes.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: CD147 polyclonal antibody detects endogenous levels of CD147 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 42 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of CD147 polyclonal antibody at 1:5000 dilution\u003cbr\u003e\n Lane 1: A375 cell membrane lysate (10ug)\u003cbr\u003e\n Lane 2: SK-OVCAR3 cell membrane lysate (4ug), Western blot (WB) analysis of CD147 polyclonal antibody at 1:2000 dilution\u003cbr\u003e\n Lane 1: EC9706 cell membrane lysate (25ug)\u003cbr\u003e\n Lane 2: A549 cell membrane lysate (25ug), Immunoprecipitation of SK-OVCAR3 cell lysates using CD147 pAb (Sepharose Bead Conjugate)#BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using CD147 pAb #NCP0177P.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Basigin; 5F7; Collagenase stimulatory factor; Extracellular matrix metalloproteinase inducer; EMMPRIN; Hepatoma-associated antigen; HAb18G; Leukocyte activation antigen M6; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor; TCSF; CD147; BSG; UNQ6505\/PRO21383\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant protein of human CD147.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43044052140211,"sku":"NCP0177P-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43044052172979,"sku":"NCP0177P-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_cb72ca91-747c-4d0e-9994-e7ce476022fd.jpg?v=1707739094","url":"https:\/\/afsbio.com\/products\/cd147-polyclonal-antibody-ncp0177p","provider":"AFSBio Inc.","version":"1.0","type":"link"}