{"product_id":"cd152-recombinant-protein-ncp0272","title":"CD152 Recombinant Protein - NCP0272","description":"\u003cp\u003eCD152 Recombinant Protein\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: NCP0272-500, NCP0272-1000\u003c\/p\u003e\n\n\u003cp\u003eSizes: 500ug, 1mg\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P16410\u003c\/p\u003e\n\n\u003cp\u003eHost: E.coli\u003c\/p\u003e\n\n\u003cp\u003eTag: His-tag\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: AAGGCAATGCATGTTGCCCAGCCGGCCGTGGTTCTGGCCAGTAGTCGTGGTATTGCAAGCTTCGTGTGCGAATATGCCAGCCCGGGTAAAGCAACCGAAGTTCGTGTGACCGTGCTGCGTCAGGCAGATAGCCAGGTTACCGAAGTGTGCGCCGCCACCTATATGATGGGTAATGAACTGACCTTCCTGGATGATAGTATCTGTACCGGTACCAGTAGTGGTAATCAGGTTAATCTGACCATTCAGGGTCTGCGCGCCATGGATACCGGCCTGTATATCTGTAAAGTTGAACTGATGTATCCGCCGCCGTATTATCTGGGCATTGGCAATGGCACCCAGATCTATGTGATTGATCCGGAACCGTGCCCGGATAGTGAT\u003c\/p\u003e\n\n\u003cp\u003eRestriction Sites: NdeI-XhoI\u003c\/p\u003e\n\n\u003cp\u003eBackground: Cytotoxic T-lymphocyte protein 4 (CTLA-4, CD152) is an Ig superfamily member that negatively regulates early T cell activation. The CTLA-4 protein is primarily expressed on T cells, including CD8+ cytotoxic T cells, CD4+ helper T cells, and CD4+\/FoxP3+ regulatory T cells. CTLA-4 protein competes with CD28 for B7.1 (CD80) and B7.2 (CD86) binding at the cell surface, which results in the down regulation of T cell activity. The activation of SHP-2 and PP2A downstream of CTLA-4 attenuates TCR signaling. Research studies indicate that CTLA4 knockout mice display lymphoproliferative disorders leading to early death, confirming the role of CTLA-4 as a negative regulator of T cells. Mutations in the corresponding CTLA4 gene are associated with multiple disorders, including insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, and type V autoimmune lymphoproliferative syndrome. Additional studies demonstrate that CTLA-4 blockade is an effective strategy for tumor immunotherapy.\u003c\/p\u003e\n\n\u003cp\u003eSoluble: PBS, 4M Urea, PH7.4\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is \u0026gt; 85% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eExpression Vector: pet-22b(+)\u003c\/p\u003e\n\n\u003cp\u003eBioWorld Molecular Weight: ~17kDa\u003c\/p\u003e\n\n\u003cp\u003eNotes: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"500ug","offer_id":42887064256691,"sku":"NCP0272-500","price":1050.83,"currency_code":"CAD","in_stock":true},{"title":"1mg","offer_id":42887064289459,"sku":"NCP0272-1000","price":1857.89,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_641490f9-b32d-469e-b1c3-c5efb7e62083.jpg?v=1702317364","url":"https:\/\/afsbio.com\/products\/cd152-recombinant-protein-ncp0272","provider":"AFSBio Inc.","version":"1.0","type":"link"}