{"product_id":"cd253-recombinant-protein-ncp0194","title":"CD253 Recombinant Protein - NCP0194","description":"\u003cp\u003eCD253 Recombinant Protein\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: NCP0194-500, NCP0194-1000\u003c\/p\u003e\n\n\u003cp\u003eSizes: 500ug, 1mg\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P50591\u003c\/p\u003e\n\n\u003cp\u003eHost: E.coli\u003c\/p\u003e\n\n\u003cp\u003eTag: His-tag\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: ACCAATGAACTGAAACAGATGCAGGATAAATATAGTAAAAGCGGTATTGCCTGCTTCCTGAAAGAAGATGATAGTTATTGGGATCCGAATGATGAAGAAAGCATGAATAGTCCGTGCTGGCAGGTGAAATGGCAGCTGCGTCAGCTGGTGCGCAAAATGATTCTGCGTACCAGCGAAGAAACCATTAGCACCGTGCAGGAAAAACAGCAGAATATTAGTCCGCTGGTTCGCGAACGCGGCCCGCAGAGAGTGGCAGCTCATATTACCGGCACCCGTGGCCGCAGTAATACCCTGAGCAGCCCGAATAGTAAAAATGAAAAAGCCCTGGGCCGCAAAATTAATAGCTGGGAAAGCAGCCGCAGTGGTCATAGCTTCCTGAGTAATCTGCATCTGCGTAATGGCGAACTGGTTATTCATGAAAAAGGCTTCTATTATATCTACAGCCAGACCTACTTCCGCTTCCAGGAAGAAATTAAAGAAAATACCAAGAACGACAAGCAGATGGTGCAGTATATCTATAAATATACCAGCTATCCGGATCCGATTCTGCTGATGAAAAGCGCCCGTAATAGTTGCTGGAGCAAAGATGCAGAATATGGTCTGTATAGTATCTATCAGGGTGGCATCTTCGAACTGAAAGAAAATGATCGTATCTTCGTGAGCGTGACCAATGAACATCTGATTGATATGGATCATGAAGCCAGCTTCTTCGGTGCATTCCTGGTTGGT\u003c\/p\u003e\n\n\u003cp\u003eRestriction Sites: NdeI-XhoI\u003c\/p\u003e\n\n\u003cp\u003eBackground: Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), also referred to as Apo2 ligand, first identified based on its sequence homology to TNF and Fas\/Apo ligand is a member of the TNF family of cytokines and either exists as a type II membrane or soluble protein. TRAIL induces apoptosis in a variety of transformed cell lines and plays a role in anti-tumor and anti-viral immune surveillance. TRAIL signals via binding with death receptors DR4 (TRAIL-R1) and DR5 (TRAIL-R2)  which can trigger apoptosis as well as NF-κB activation. Death domains on these receptors leads to the recruitment of a death-induced signaling complex (DISC) leading to caspase-8 and subsequent caspase-3 activation. In addition, TRAIL binds with decoy receptors DcR1 (TRAIL-R3) and DcR2 (TRAIL-R4, TRUNDD) which lack the functional cytoplasmic death domain antagonizing TRAIL-induced apoptosis. Osteoprotegerin (OPG) has also been identified as receptor capable of inhibiting TRAIL-induced apoptosis. The selectivity of soluble TRAIL at triggering apoptosis in transformed cells as compared to normal cells has led to its investigation as a potential cancer therapeutic.\u003c\/p\u003e\n\n\u003cp\u003eSoluble: PBS, 4M Urea, PH7.4\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is \u0026gt; 85% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eExpression Vector: pet-22b(+)\u003c\/p\u003e\n\n\u003cp\u003eBioWorld Molecular Weight: ~27kDa\u003c\/p\u003e\n\n\u003cp\u003eNotes: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"500ug","offer_id":42887058555059,"sku":"NCP0194-500","price":1050.83,"currency_code":"CAD","in_stock":true},{"title":"1mg","offer_id":42887058587827,"sku":"NCP0194-1000","price":1857.89,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_0de6f104-55ea-4f4e-acff-6eefe496e6d8.jpg?v=1702317204","url":"https:\/\/afsbio.com\/products\/cd253-recombinant-protein-ncp0194","provider":"AFSBio Inc.","version":"1.0","type":"link"}