{"product_id":"cd282-recombinant-protein-ncp0375","title":"CD282 Recombinant Protein - NCP0375","description":"\u003cp\u003eCD282 Recombinant Protein\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: NCP0375-500, NCP0375-1000\u003c\/p\u003e\n\n\u003cp\u003eSizes: 500ug, 1mg\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O60603\u003c\/p\u003e\n\n\u003cp\u003eHost: E.coli\u003c\/p\u003e\n\n\u003cp\u003eTag: His-tag\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: ESSNQASLSCDRNGICKGSSGSLNSIPSGLTEAVKSLDLSNNRITYISNSDLQRCVNLQALVLTSNGINTIEEDSFSSLGSLEHLDLSYNYLSNLSSSWFKPLSSLTFLNLLGNPYKTLGETSLFSHLTKLQILRVGNMDTFTKIQRKDFAGLTFLEELEIDASDLQSYEPKSLKSIQNVSHLILHMKQHILLLEIFVDVTSSVECLELRDTDLDTFHFSELSTGETNSLIKKFTFRNVKITDESLFQVMKLLNQISGLLELEFDDCTLNGVGNFRASDNDRVIDPGKVETLTIRRLHIPRFYLFYDLSTLYSLTERVKRIt VENSKVFLVPCLLSQHLKSLEYLDLSENLMVEEYLKNSACEDAWPSLQTLILRQNHLASLEKTGETLLTLKNLTNIDISKNSFHSMPETCQWPEKMKYLNLSSTRIHSVTGCIPKTLEILDVSNNNLNLFSLNLPQLKELYISRNKLMTLPDASLLPMLLVLKISRNAITTFSKEQLDSFHTLKTLEAGGNNFICSCEFLSFTQEQQALAKVLIDWPANYLCDSPSHVRGQQVQDVRLSVSECHRT\u003c\/p\u003e\n\n\u003cp\u003eRestriction Sites: NdeI-XhoI\u003c\/p\u003e\n\n\u003cp\u003eBackground: Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses. TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll\/Interleukin-1 receptor (TIR) domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like\/TIR-associated protein (MAL\/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.\u003cbr\u003e\nTLR2 is expressed on the surface of monocytes and macrophages and can heterodimerize with TLR1 or TLR6, enabling responses to a variety of pathogen-associated molecular patterns including lipopetides and peptidoglycan.\u003c\/p\u003e\n\n\u003cp\u003eSoluble: PBS, 4M Urea, PH7.4\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is \u0026gt; 85% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eExpression Vector: pet-22b(+)\u003c\/p\u003e\n\n\u003cp\u003eBioWorld Molecular Weight: ~68kDa\u003c\/p\u003e\n\n\u003cp\u003eNotes: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"500ug","offer_id":42887072710835,"sku":"NCP0375-500","price":1050.83,"currency_code":"CAD","in_stock":true},{"title":"1mg","offer_id":42887072743603,"sku":"NCP0375-1000","price":1857.89,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_018d997f-648e-4bbb-8686-82cee6c31575.jpg?v=1702317546","url":"https:\/\/afsbio.com\/products\/cd282-recombinant-protein-ncp0375","provider":"AFSBio Inc.","version":"1.0","type":"link"}