{"product_id":"cd31-polyclonal-antibody-ncp0199p","title":"CD31 Polyclonal Antibody-NCP0199P","description":"\u003cp\u003eCD31 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: NCP0199P-50, NCP0199P-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P16284\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:5000~1:10000\u003cbr\u003e\nIP  1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis. CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members. The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: CD31 polyclonal antibody detects endogenous levels of CD31 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 130 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of CD31 polyclonal antibody at 1:5000 dilution\u003cbr\u003e\n Lane 1: Jurkat whole cell lysate (30ug)\u003cbr\u003e\n Lane 2: THP-1 whole cell lysate (30ug)\u003cbr\u003e\n Lane 3: A549 whole cell lysate (30ug), Immunoprecipitation of THP-1 cell membrane lysates using CD31 pAb (Sepharose Bead Conjugate)#BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using CD31 pAb #NCP0199P.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD31; PECAM1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant protein of human CD31.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43044042277043,"sku":"NCP0199P-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43044042309811,"sku":"NCP0199P-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_e458ecb0-54d0-438d-9c56-9f1ab4c27942.jpg?v=1707738962","url":"https:\/\/afsbio.com\/products\/cd31-polyclonal-antibody-ncp0199p","provider":"AFSBio Inc.","version":"1.0","type":"link"}