{"product_id":"cd344-recombinant-protein-ncp0299","title":"CD344 Recombinant Protein - NCP0299","description":"\u003cp\u003eCD344 Recombinant Protein\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: NCP0299-500, NCP0299-1000\u003c\/p\u003e\n\n\u003cp\u003eSizes: 500ug, 1mg\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9ULV1\u003c\/p\u003e\n\n\u003cp\u003eHost: E.coli\u003c\/p\u003e\n\n\u003cp\u003eTag: His-tag\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: TTCGGCGATGAAGAAGAACGTCGCTGCGATCCGATTCGTATTAGTATGTGCCAGAATCTGGGTTATAATGTTACCAAAATGCCGAATCTGGTGGGCCATGAACTGCAGACCGATGCAGAACTGCAGCTGACCACCTTCACCCCGCTGATTCAGTATGGCTGTAGTAGTCAGCTGCAGTTCTTCCTGTGCAGTGTGTATGTGCCGATGTGCACCGAAAAAATTAATATTCCGATTGGTCCGTGTGGTGGCATGTGCCTGAGCGTGAAACGTCGTTGCGAACCGGTTCTGAAAGAATTCGGCTTCGCCTGGCCGGAAAGCCTGAATTGTAGTAAATTCCCGCCGCAGAATGATCATAATCACATGTGCATGGAAGGTCCGGGTGATGAAGAAGTTCCGCTGCCGCATAAAACACCTATTCAGCCGGGTGAAGAATGTCATAGTGTGGGCACCAATAGCGATCAGTATATCTGGGTTAAACGCAGTCTGAATTGCGTTCTGAAATGTGGTTATGATGCAGGCCTGTATAGC\u003c\/p\u003e\n\n\u003cp\u003eRestriction Sites: NdeI-XhoI\u003c\/p\u003e\n\n\u003cp\u003eBackground: Frizzled-4 is a 537 amino acid protein encoded by the human gene FZD4. Frizzled-4 acts as a receptor for Wnt proteins. Most frizzled receptors are coupled to the b-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of b-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Frizzled-4 may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and\/or in differentiated tissues. Frizzled-4 also plays a critical role in retinal angiogenesis. Frizzled-4 is virtually ubiquitously expressed with greatest amounts found in adult heart, skeletal muscle, ovary, and fetal kidney\u003c\/p\u003e\n\n\u003cp\u003eSoluble: PBS, 4M Urea, PH7.4\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is \u0026gt; 85% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eExpression Vector: pet-22b(+)\u003c\/p\u003e\n\n\u003cp\u003eBioWorld Molecular Weight: ~24kDa\u003c\/p\u003e\n\n\u003cp\u003eNotes: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"500ug","offer_id":42887066157235,"sku":"NCP0299-500","price":1050.83,"currency_code":"CAD","in_stock":true},{"title":"1mg","offer_id":42887066190003,"sku":"NCP0299-1000","price":1857.89,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_078abd5f-feb9-4be7-94b6-6db64d49408c.jpg?v=1702317411","url":"https:\/\/afsbio.com\/products\/cd344-recombinant-protein-ncp0299","provider":"AFSBio Inc.","version":"1.0","type":"link"}