{"product_id":"cd365-recombinant-protein-ncp0329","title":"CD365 Recombinant Protein - NCP0329","description":"\u003cp\u003eCD365 Recombinant Protein\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: NCP0329-500, NCP0329-1000\u003c\/p\u003e\n\n\u003cp\u003eSizes: 500ug, 1mg\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q96D42\u003c\/p\u003e\n\n\u003cp\u003eHost: E.coli\u003c\/p\u003e\n\n\u003cp\u003eTag: His-tag\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: SVKVGGEAGPSVTLPCHYSGAVTSMCWNRGSCSLFTCQNGIVWTNGTHVTYRKDTRYKLL\u003cbr\u003e\nGDLSRRDVSLTIENTAVSDSGVYCCRVEHRGWFNDMKIt VSLEIVPPKVTTTPIVTt VPT\u003cbr\u003e\nVTt VRTSTt VPTTTt VPMTt VPTTt VPTTMSIPTTTt VLTTMt VSTTTSVPTTTSIPTTT\u003cbr\u003e\nSVPVTTt VSTFVPPMPLPRQNHEPVATSPSSPQPAETHPTTLQGAIRREPTSSPLYSYTT\u003cbr\u003e\nDGNDt VTESSDGLWNNNQTQLFLEHSLLTANTTKG\u003c\/p\u003e\n\n\u003cp\u003eRestriction Sites: NdeI-XhoI\u003c\/p\u003e\n\n\u003cp\u003eBackground: T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-1 (HAVCR1 (hepatitis A virus cellular receptor 1), KIM-1 (kidney injury molecule-1) was originally identified as a receptor for hepatitis A virus. TIM-1 also acts as a costimulatory receptor on T cells and following activation, associates with the TCR complex to upregulate signaling and cytokine production. Another TIM family member, TIM-4, is expressed by antigen presenting cells and is a ligand for TIM-1. TIM-1 expressed by Th1 and Th17 cells was also recently shown to interact with P-selectin to mediate T cell trafficking during inflammation and autoimmune disease. NKT cells also express TIM-1, and engagement of TIM-1 on NKT cells leads to increased production of IL-4, but decreased production of IFN-gamma. TIM-1 is also a receptor for phosphatidylserine exposed by cells undergoing apoptosis. Detection of phosphatidylserine by TIM-1 expressed on NKT cells results in activation, proliferation, and cytokine production. Expression of TIM-1 on regulatory B cells is required for optimal production of IL-10. Mice lacking the TIM-1 mucin domain have decreased production of IL-10 by regulatory B cells, hyperactive T cells, increased levels of inflammatory cytokines, and enhanced severity of autoimmune disease. In addition, TIM-1 polymorphisms are associated with susceptibility to atopic diseases including asthma. Finally, expression of TIM-1 is increased in renal tubular epithelial cells following kidney injury.\u003c\/p\u003e\n\n\u003cp\u003eSoluble: PBS, 4M Urea, PH7.4\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is \u0026gt; 85% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eExpression Vector: pet-22b(+)\u003c\/p\u003e\n\n\u003cp\u003eBioWorld Molecular Weight: ~34kDa\u003c\/p\u003e\n\n\u003cp\u003eNotes: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"500ug","offer_id":42887068450995,"sku":"NCP0329-500","price":1050.83,"currency_code":"CAD","in_stock":true},{"title":"1mg","offer_id":42887068483763,"sku":"NCP0329-1000","price":1857.89,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_692d78a0-3dc7-4133-8e52-06def885ad89.jpg?v=1702317470","url":"https:\/\/afsbio.com\/products\/cd365-recombinant-protein-ncp0329","provider":"AFSBio Inc.","version":"1.0","type":"link"}