{"product_id":"cd3eap-e474-polyclonal-antibody-bs3852","title":"CD3EAP (E474) Polyclonal Antibody-BS3852","description":"\u003cp\u003eCD3EAP (E474) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS3852-50, BS3852-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O15446\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: IHC\u003c\/p\u003e\n\n\u003cp\u003eBackground: CD3EAP, also known as ASE1, CAST or PAF49, is a 510 amino acid protein that localizes to the nucleus and belongs to the eukaryotic RPA34 RNA polymerase subunit family. Existing as two alternatively spliced isoforms, CD3EAP functions as a component of the Pol I complex, specifically exhibiting DNA-dependent RNA polymerase activity and effectively catalyzing the the conversion of a nucleoside triphosphate into a diphosphate, thereby transcribing DNA into RNA. Isoform 2 of CD3EAP may be a component of the T cell receptor (TCR) complex and is subject to tyrosine phosphorylation in response to TCR stimulation. Both isoforms of CD3EAP are subject to DNA damage-dependent phosphorylation, probably by ATM or ATR.\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: CD3EAP (E474) polyclonal antibody detects endogenous levels of CD3EAP protein.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Immunohistochemistry (IHC) analyzes of CD3EAP (E474) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: ~ 55, 75 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: DNA-directed RNA polymerase I subunit RPA34; A34.5; Antisense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CAST; CD3E-associated protein; RNA polymerase I-associated factor PAF49; ASE1; CAST; PAF49\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 451-500 of Human CD3EAP.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42949469634739,"sku":"BS3852-50","price":254.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42949469667507,"sku":"BS3852-100","price":442.75,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_320cba98-f1cd-4f10-8570-8dacfd728e5e.jpg?v=1704970083","url":"https:\/\/afsbio.com\/products\/cd3eap-e474-polyclonal-antibody-bs3852","provider":"AFSBio Inc.","version":"1.0","type":"link"}