{"product_id":"cd3eap-polyclonal-antibody-ra23052","title":"CD3EAP Polyclonal Antibody - RA23052","description":"\u003cp\u003eCD3EAP Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RA23052-50, RA23052-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: caution:It is not known whether the so-called human ASE1 and human CAST proteins represent two sides of a single gene product with sharply different functional characteristics. Experiments done with the mouse homolog protein are in favor of an implication of this gene in rRNA transcription instead of T-cell receptor signaling.,function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.,function:Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.,miscellaneous:It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in mouse suggesting an important biologic function.,PTM:Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes.,PTM:Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the eukaryotic RPA34 RNA polymerase subunit family.,subcellular location:Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes.,subunit:Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1A thereby associates with the SL1 complex. Interacts with UBTF. Interacts with POLR1E\/PRAF1 through its N-terminal region (By similarity) . Isoform 2 interacts with CD3E.,\u003c\/p\u003e\n\n\u003cp\u003eCondition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eStorage Instructions: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eRecommended dilutions: Western Blot: 1\/500 - 1\/2000. Immunohistochemistry: 1\/100 - 1\/300. ELISA: 1\/40000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: CD3EAP; ASE1; CAST; PAF49; DNA-directed RNA polymerase I subunit RPA34; A34.5; Antisense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CAST; CD3E-associated protein; RNA polymerase I-associated factor PAF49\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB; IHC-p; IF (paraffin section) ; ELISA\u003c\/p\u003e\n\n\u003cp\u003eSpecies Cross-Reactivity: Human; Rat; Mouse\u003c\/p\u003e\n\n\u003cp\u003eGeneID (Human): 10849\u003c\/p\u003e\n\n\u003cp\u003eProtein MW (KDa): 55\u003c\/p\u003e\n\n\u003cp\u003eSWISS: O15446\u003c\/p\u003e\n\n\u003cp\u003eSource: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Reed Bio","offers":[{"title":"50μL","offer_id":45121185906867,"sku":"RA23052-50","price":336.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45121185939635,"sku":"RA23052-100","price":448.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/logo_e1b3a696-daf2-47c9-8ed0-458da20f3322.png?v=1773344959","url":"https:\/\/afsbio.com\/products\/cd3eap-polyclonal-antibody-ra23052","provider":"AFSBio Inc.","version":"1.0","type":"link"}