{"product_id":"cd4-rabbit-polyclonal-antibody-es1909","title":"CD4 Rabbit Polyclonal Antibody - ES1909","description":"\u003cp\u003eCD4 Rabbit Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES1909-50, ES1909-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010],\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4\/Leu-3, CD antigen CD4\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human CD4. AA range:401-450\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eTarget: CD4\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: CD4 Polyclonal Antibody detects endogenous levels of CD4 protein.\u003c\/p\u003e\n\n\u003cp\u003eDilution: Western Blot: 1\/500 - 1\/2000. IHC-p: 1:100-300 ELISA: 1\/20000. IF 1:100-300 Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml,\u003c\/p\u003e\n\n\u003cp\u003eFormlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eExpression: Highly expressed in T-helper cells, The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes, CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells,\u003c\/p\u003e\n\n\u003cp\u003eObserved Band (KD): 51kD\u003c\/p\u003e\n\n\u003cp\u003eFields: Viral life cycle - HIV-1, Cytokine-cytokine receptor interaction, Cell adhesion molecules, Antigen processing and presentation, Hematopoietic cell lineage, Th1 and Th2 cell differentiation, Th17 cell differentiation, T cell receptor signaling pathway, Yersinia infection, Human T-cell leukemia virus 1 infection, Human immunodeficiency virus 1 infection, PD-L1 expression and PD-1 checkpoint pathway in cancer, Primary immunodeficiency\u003c\/p\u003e\n\n\u003cp\u003eFunction: Function: Accessory protein for MHC class-II antigen\/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts\u003cbr\u003e\nMiscellaneous: Primary receptor for HIV-1\u003cbr\u003e\nOnline Information: CD4 entry,PTM: Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts\u003cbr\u003e\nSimilarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain\u003cbr\u003e\nSimilarity: Contains 3 Ig-like C2-type (immunoglobulin-like) domains\u003cbr\u003e\nSubcellular Location: Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum\u003cbr\u003e\nSubunit: Associates with LCK. Binds to HIV-1 gp120 and to P4HB\/PDI and upon HIV-1 binding to t\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: Cell membrane, Single-pass type I membrane protein Localizes to lipid rafts (PubMed:12517957, PubMed:9168119) Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eGene Name: CD4\u003c\/p\u003e\n\n\u003cp\u003eProtein Name: T-cell surface glycoprotein CD4\u003c\/p\u003e\n\n\u003cp\u003eMouse Gene ID: 12504\u003c\/p\u003e\n\n\u003cp\u003eMouse SwissProt Number: P06332\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":46315874943155,"sku":"ES1909-50","price":186.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":46315874975923,"sku":"ES1909-100","price":372.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/elk_antibodies.jpg?v=1793190497","url":"https:\/\/afsbio.com\/products\/cd4-rabbit-polyclonal-antibody-es1909","provider":"AFSBio Inc.","version":"1.0","type":"link"}