{"product_id":"cd45-phospho-y1216-polyclonal-antibody-bs64566","title":"CD45 (Phospho-Y1216) Polyclonal Antibody-BS64566","description":"\u003cp\u003eCD45 (Phospho-Y1216) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS64566-50, BS64566-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P08575\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing. The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit\/bind substrates. CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling. Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: CD45 (Phospho-Y1216) polyclonal antibody detects endogenous levels of CD45 protein only when phosphorylated at Tyr1216.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 180 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western Blot (WB) analysis of CD45 (Phospho-Y1216) polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLane A: HEK293T whole cell lysate\u003cbr\u003e\nLane B: MCF-7 whole cell lysate\u003cbr\u003e\nLane C: PC12 whole cell lysate\u003cbr\u003e\nLane D: AML-12 whole cell lysate\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; PTPRC; CD45\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic phosphopeptide derived from human CD45 around the phosphorylation site of Tyrosine 1216.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42923463475379,"sku":"BS64566-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42923463508147,"sku":"BS64566-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_6b41b2d4-fb2b-497a-9e40-d8a98a7a552c.jpg?v=1703849973","url":"https:\/\/afsbio.com\/products\/cd45-phospho-y1216-polyclonal-antibody-bs64566","provider":"AFSBio Inc.","version":"1.0","type":"link"}