{"product_id":"cd71-rabbit-monoclonal-antibody-mb66397","title":"CD71 Rabbit Monoclonal Antibody-MB66397","description":"\u003cp\u003eCD71 Rabbit Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB66397-50, MB66397-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P02786\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB (1\/500 - 1\/1000), IF\/ICC (1\/50 - 1\/100), IP (1\/10 - 1\/50)\u003c\/p\u003e\n\n\u003cp\u003eBackground: Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes .\u003cbr\u003e\nEndosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity).\u003cbr\u003e\nA second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake.\u003cbr\u003e\nActs as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway .\u003cbr\u003e\nWhen dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion.\u003cbr\u003e\nWhen dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2.By Similarity3 Publications\u003cbr\u003e\n(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was purified by immunogen affinity chromatography.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of CD71 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 90 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of CD71 expression in K562 (A), Hela (B) whole cell lysates., Immunofluorescent analysis of CD71 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Transferrin receptor protein 1; TR; TfR; TfR1; Trfr; T9; p90; CD71\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant protein of human Transferrin Receptor\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42987096572083,"sku":"MB66397-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42987096604851,"sku":"MB66397-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_8e564900-244e-486d-86ba-0e171145c95a.jpg?v=1706009143","url":"https:\/\/afsbio.com\/products\/cd71-rabbit-monoclonal-antibody-mb66397","provider":"AFSBio Inc.","version":"1.0","type":"link"}