{"product_id":"cd71-tfr-polyclonal-antibody-ncp0225p","title":"CD71\/TfR Polyclonal Antibody-NCP0225P","description":"\u003cp\u003eCD71\/TfR Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: NCP0225P-50, NCP0225P-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P02786\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP,  IF\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:2000~1:5000\u003cbr\u003e\nIF  1:50~1:200\u003cbr\u003e\nIP  1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Transferrin receptor 1 (CD71, TFRC) is a type II transmembrane receptor and carrier protein responsible for the uptake of cellular iron through receptor-mediated endocytosis. Neutral pH at the cell surface promotes binding of the iron-binding glycoprotein transferrin (Tf) to the CD71 receptor. The receptor-ligand complex enters the cell through receptor-mediated endocytosis and is internalized into an endosome. Relatively lower endosomal pH leads to a change in the local charge environment surrounding the iron-transferrin binding site and results in the release of iron. The receptor-ligand complex is recycled to the cell surface where transferrin dissociates from the CD71 receptor. Ubiquitously expressed transferrin receptor is continuously recycled and undergoes clathrin-mediated endocytosis regardless of ligand binding state. The interaction between receptor and ligand has been studied in detail. The helical domain of CD71 directly interacts with the transferrin C-lobe and induces a conformation change in Tf to facilitate the transport process. Interaction between the receptor CD71 and transferrin is mediated by the membrane protein hemochromatosis (HFE). HFE binds the α-helical domain of CD71, blocking formation of the CD71-transferrin complex and inhibiting iron uptake. In addition to binding transferrin, CD71 also interacts with H-ferritin at the cell surface and transports this intracellular iron storage protein to cellular endosomes and lysosomes. Additional studies indicate that the transferrin receptor is an evolutionarily conserved receptor for a number of arenaviruses and at least one retrovirus. Aberrant expression of CD71 is seen in a number of cancers, including thyroid carcinomas, lymphomas, and T-lineage leukemias, suggesting a possible therapeutic role for targeted inhibition of the transferrin receptor.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: CD71\/TfR polyclonal antibody detects endogenous levels of CD71\/TfR protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 90 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of CD71\/TfR polyclonal antibody at 1:2000 dilution\u003cbr\u003e\n Lane 1: A549 whole cell lysate (30ug)\u003cbr\u003e\n Lane 2: L02 whole cell lysate (30ug), Immunofluorescence analysis of A549 cells using CD71\/TfR pAb at dilution of 1:200., Immunoprecipitation of A549 cell membrane lysates using CD71\/TfR pAb (Sepharose Bead Conjugate)#BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using CD71\/TfR pAb.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Transferrin receptor protein 1; TR; TfR; TfR1; Trfr; T9; p90; CD_antigen: CD71; Transferrin receptor protein 1, serum form; sTfR; TFRC\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant protein of human CD71\/TfR.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43044051615923,"sku":"NCP0225P-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43044051648691,"sku":"NCP0225P-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_076ce144-3fc3-4267-a06d-ffeb5bbbb7b5.jpg?v=1707739082","url":"https:\/\/afsbio.com\/products\/cd71-tfr-polyclonal-antibody-ncp0225p","provider":"AFSBio Inc.","version":"1.0","type":"link"}