{"product_id":"cdc6-phospho-s54-polyclonal-antibody-bs94031","title":"Cdc6 (Phospho-S54) Polyclonal Antibody-BS94031","description":"\u003cp\u003eCdc6 (Phospho-S54) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS94031-50, BS94031-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q99741(Human)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\/IF\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:1,000-1:2,000\u003cbr\u003e\nICC:1:50-1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division control (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein Tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory Tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4\/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (x complex) which ubiquitinates cyclin B, resulting in cyclin B\/Cdk complex degradation.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Cdc6 (Phospho-S54) polyclonal antibody detects endogenous levels of Cdc6 protein only when phosphorylated at S54.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 63 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Phospho-Cdc6(S54) on Jurkat cells lysates using anti-Phospho-Cdc6(S54) antibody at 1\/1,000 dilution., ICC staining Phospho-Cdc6(S54) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: HsCdc18, HsCDC6, CDC6, Cdc 18L, Cdc 6, CDC18 (cell division cycle 18, S.pombe, homolog) like, CDC18 (S.pombe), Cdc18 related protein, CDC18(S.pombe), Cdc18-related protein, Cdc18L, cdc6, CDC6 cell division cycle 6 homolog, CDC6 related protein, CDC6-related protein, CDC6, Cdc6p, CELL CYCLE CONTROLLER CDC6, Cell division control protein 6, Cell division control protein 6 homolog, Cell division cycle 6 homolog (S. cerevisiae), Cell division cycle 6 homolog, Cell division cycle 6, S. cerevisiae, homolog of, HsCDC 6, HsCDC18, HsCDC6, p62, p62(cdc 6), p62(cdc6),\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser54 of human Cdc6.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42987065704627,"sku":"BS94031-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42987065737395,"sku":"BS94031-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_5c6d9888-23ec-4f49-9d9f-85d5ef502214.jpg?v=1706008320","url":"https:\/\/afsbio.com\/products\/cdc6-phospho-s54-polyclonal-antibody-bs94031","provider":"AFSBio Inc.","version":"1.0","type":"link"}