{"product_id":"cdk1-polyclonal-antibody-bs90278","title":"CDk1 Polyclonal Antibody-BS90278","description":"\u003cp\u003eCDk1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90278-50, BS90278-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P06493\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC IHC FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500\u003cbr\u003e\nICC:1:200\u003cbr\u003e\nIHC:1:200\u003cbr\u003e\nFC:1:100-1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Cdk1 is a small protein (approximately 34 kilodaltons), and is highly conserved. Cdk1 is comprised mostly by the bare protein kinase motif, which other protein kinases share. Cdk1, like other kinases, contains a cleft in which ATP fits. When bound to its cyclin partners, Cdk1 phosphorylation leads to cell cycle progression. Given its essential role in cell cycle progression, Cdk1 is highly regulated. Most obviously, Cdk1 is regulated by its binding with its cyclin partners. Cyclin binding alters access to the active site of Cdk1, allowing for Cdk1 activity; furthermore, cyclins impart specificity to Cdk1 activity. At least some cyclins contain a hydrophobic patch which may directly interact with substrates, conferring target specificity. Furthermore, cyclins can target Cdk1 to particular subcellular locations.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Peptide affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: CDk1 polyclonal antibody detects endogenous levels of CDk1 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 34 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of CDk1 on different cell lysates using anti-CDk1 antibody at 1\/500 dilution. \u003cbr\u003e\n Positive control: \u003cbr\u003e\n Lane 1: MCF-7 \u003cbr\u003e\n Lane 2: Jurkat \u003cbr\u003e\n Lane 3: PC12 \u003cbr\u003e\n Lane 4: HepG2 \u003cbr\u003e\n Lane 5: Hela\u003cbr\u003e\n Lane 6: NIH\/3T3 \u003cbr\u003e\n Lane 7: Mouse liver \u003cbr\u003e\n Lane 8: SKBR3, ICC staining CDk1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: CDK1, Cdc 2, Cdc2, CDC28A, CDK 1, CDK1, CDK1, CDKN1, CELL CYCLE CONTROLLER CDC2, Cell division control protein 2, Cell division control protein 2 homolog, Cell division cycle 2 G1 to S and G2 to M, Cell division protein kinase 1, Cell Divsion Cycle 2 Protein, Cyclin Dependent Kinase 1, Cyclin-dependent kinase 1, DKFZp686L20222, MGC111195, p34 Cdk1, p34 protein kinase, P34CDC2,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: peptide\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42979798450355,"sku":"BS90278-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42979798483123,"sku":"BS90278-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_f0b2e30a-8006-45f2-8595-8c8dd39add24.jpg?v=1705752727","url":"https:\/\/afsbio.com\/products\/cdk1-polyclonal-antibody-bs90278","provider":"AFSBio Inc.","version":"1.0","type":"link"}