{"product_id":"cdk4-monoclonal-antibody-mb66514","title":"CDK4 Monoclonal Antibody - MB66514","description":"\u003cp\u003eCDK4 Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μl, 100μl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB66514-50, MB66514-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: Cyclin-dependent kinase activity is regμlated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip\/Kip or INK family of proteins. The inactive ternary complex of CDK4\/cyclin D and p27 Kip1\/Cip1 requires extracellμlar mitogenic stimμli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase. The active complex of CDK4\/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1\/S-phase gene expression. In HeLa cells, upon UV irradiation, upregμlation of p16 INK4A association with CDK4\/cyclin D3 leads to a G2 delay, implicating CDK4\/cyclin D3 activity in progression through the G2-phase of the cell cycle.\u003c\/p\u003e\n\n\u003cp\u003eCategory: Primary Antibody\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3\u003c\/p\u003e\n\n\u003cp\u003eClonality: Monoclonal\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodification\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Purified recombinant human CDK4 protein fragments expressed in E.coli.\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB (1\/500 - 1\/1000)\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was purified by immunogen affinity chromatography.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of CDK4 protein.\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: ~ 33 kDa\u003c\/p\u003e\n\n\u003cp\u003ePathway: Immune checkpoint signaling in the Tumor Microenvironment\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSwissProt: P11802\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Bioworld","offers":[{"title":"50μl","offer_id":45946406568115,"sku":"MB66514-50","price":274.5,"currency_code":"CAD","in_stock":true},{"title":"100μl","offer_id":45946406600883,"sku":"MB66514-100","price":457.5,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Bioworld_Antibody_Image_6ca33534-2486-4e91-ac84-9e0d75778258.jpg?v=1788363539","url":"https:\/\/afsbio.com\/products\/cdk4-monoclonal-antibody-mb66514","provider":"AFSBio Inc.","version":"1.0","type":"link"}