{"product_id":"cgn-polyclonal-antibody-bs72612","title":"CGN Polyclonal Antibody-BS72612","description":"\u003cp\u003eCGN Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50 µl, 100 µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS72612-50, BS72612-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9P2M7\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eCGN Polyclonal Antibody-BS72612\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:200 - 1:2000  |  IHC,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Probably plays a role in the formation and regulation of the tight junction (TJ paracellular permeability barrier.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Polyclonal Antibodies\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 160kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using CGN antibody at 1:1000 dilution.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 90s., Immunohistochemistry of paraffin-embedded Human colon carcinoma using CGN Rabbit pAb at dilution of 1:100.Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: CGN; cingulin\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human CGN(NP_065821.1).\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42968435228851,"sku":"BS72612-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42968435261619,"sku":"BS72612-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_7fc68eca-a59c-410f-bbca-21180ac3cd45.jpg?v=1705427758","url":"https:\/\/afsbio.com\/products\/cgn-polyclonal-antibody-bs72612","provider":"AFSBio Inc.","version":"1.0","type":"link"}