Chicken Anti-Biotin Ligase/BioID2 Polyclonal Antibody IgY 100μg (Polyclonal)

Chicken Anti-Biotin Ligase/BioID2 Polyclonal Antibody IgY 100μg (Polyclonal)

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Form: Purified Immune IgY from chicken eggs

Specificity: Reacts with native A. aeolicus biotin ligase and the R40G mutant used in BioID2

Buffer/Composition: 0.01% sodium azide, glycerol (50% v/v), PBS (50% v/v)

Volume: 100 microliters (μl)

Concentration: 10 mg/ml

Storage: 

  • Short Term (<6 months), 4°C
  • Long Term (>6 months to 1 year), -20°C (avoid repeated freeze/thaw)

 Working Dilutions:

  • Western Blot: 1:40,000 
  • Immunofluorescence: 1:5,000

Shipping: Ice packs

 

Images:

 

 

References:

  1. BioID: Roux KJ, Kim DI, Raida M, Burke BJ. A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. Cell Biol. 196(6):801-10, 2012
  2. BioID2: Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ. An improved smaller biotin ligase for BioID proximity labeling. Mol Biol Cell. 27(8):1185-96, 2016
  3. Review: Kim DI, Roux KJ. Filling the Void: Proximity-Based Labeling of Proteins in Living Cells. Trends in Cell Biology (in press) 2016