Form: Purified Immune IgY from chicken eggs
Specificity: Reacts with native A. aeolicus biotin ligase and the R40G mutant used in BioID2
Buffer/Composition: 0.01% sodium azide, glycerol (50% v/v), PBS (50% v/v)
Volume: 100 microliters (μl)
Concentration: 10 mg/ml
Storage:
- Short Term (<6 months), 4°C
- Long Term (>6 months to 1 year), -20°C (avoid repeated freeze/thaw)
Working Dilutions:
- Western Blot: 1:40,000
- Immunofluorescence: 1:5,000
Shipping: Ice packs
Images:
References:
- BioID: Roux KJ, Kim DI, Raida M, Burke BJ. A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. Cell Biol. 196(6):801-10, 2012
- BioID2: Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ. An improved smaller biotin ligase for BioID proximity labeling. Mol Biol Cell. 27(8):1185-96, 2016
- Review: Kim DI, Roux KJ. Filling the Void: Proximity-Based Labeling of Proteins in Living Cells. Trends in Cell Biology (in press) 2016